| Nr. 4 - 4.
Quartal 1999
Fucoidin:
Intravital-microscopical Study to Investigate the Effect of a Blockade
of Selectins in a Xenogeneic Liver Perfusion Model The massive alteration of the microcirculation of a transplanted xenograft is caused by the activation of its endothelial cell layer, accumulation of leukocytes and complement factors (C). The influence of the selectin-dependent leukocyte-endothelial cell interaction on the hyperacute xenograft rejection (HXR) remains unclear. The aim of this study was to investigate the cellular mechanism during HXR regulated by selectins. Intravital microscopy allows the direct visualization of leukocyte- endothelial cell interaction in the early phase of HXR in xenogeneic liver hemoperfusion (XLP). Within the first minutes of XLP the sinusoidal blood flow decreases, simultaneously, portal pressure rises and bile production stops. Blocking of the selectins using Fucoidin leads to an improvement of hemodynamic parameters comparable to isogeneic values without any influence on the bile production. This observation suggests an important role of a selectin-dependent cellular mechanism in the development of xenogeneic organ failure besides pnAb and C. Key words: Xenogeneic liver perfusion, selectins, fucoidin, intravital microscopy Dr. F.
M.-J. Wagner
|
