Nr. 3 - 3. Quartal 1998

"Combi-Effect" after Xenogen Discordant Heart-Lung Transplantation

C. Kelm, J. Adams, J. Buhr, K. Henneking, K. Schwemmle

The purpose of this study was to investigate whether the "Combi-Effect" after combined xenogeneic discordant heart-lung- transplantation (HLTx) is specific for the transplanted lung tissue or not. In order to answer this question we compared the heterotopic HLTx (n=5) with a double heart transplantation (DHTx, n=5) and a combined heart-kidney-transplantation (HKTx, n=5) in a guinea-pig to rat model. As a control we took the isolated xenogenic discordant heart-transplantation (HTx, n=5).

Apart from the heart transplant survival we determined the concentrations of histamine, CH-50, IgG, IgM, leucocytes and thrombocytes in the blood. At time of rejection all tissues were examined histologically and immunohistologically (ED-11, IgG, IgM, OX-39, W3-13, ED-1, NkR-P1, OX-19).

We could achieve a significant prolongation of the heart transplant survival time by combined HLTx compared to HTx (25’ to 12’, p <0,01). dhtx showed no effect (7’53’’ to 11’27’’). but after hktx the cardiac survival was even longer than after htx and hltx (62,8’ to 12’ and 25’, p < 0,01).

CH-50 showed significant lower concentrations after HLTx (180 U/l) and HKTx (178 U/l) than after HTx (260 U/l, p <0,01) and a control group (405 u/l). immunohistologically c3 revealed a lower deposition in the rejected heart transplants after combined hltx/hktx than after isolated htx.

The „Combi-Effect„ is stronger after xenogeneic discordant HKTx than after xenogeneic discordant HLTx. He is not specific for the lung tissue.

Keywords:

Xenogenic discordant transplantation, heart transplantation, heart-lung-transplantation

Kurztitel:

Kelm, C. et al. (1998) TxMed 3:157

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