Nr. 2 - 2. Quartal 1994

Electronmicroscopical Changes of Sinusoidal Lining Cells after Orthotopic Rat Liver Transplantation

T. Weber et al.

Kupffer cells and sinusoidal endothelial cells constitute the main population of sinusoidal lining cells. In this study we have examined electronmicroscopical changes of sinusoidal endothelial cells (SEC) and their enclosing anatomical structure, the space of Disse (SD), during allograft rejection. Orthotopic rat liver transplantation was done using suture techniques. The main feature of acute rejection (DA to Lewis, 10 days after transplantation) was a widening of the SD containing erythrocytes (peliosis) and infiltrating cells. Endothelial sprouts into the SD formed neocavities (neoangiogenesis). Sinusoidal ultrastructure resembled a capillarisation of sinusoids although no continous basement membrane was present. Vacuole-like structures and enlarged nuclei and mitochondria were seen in sinusoidal as well as perisinusoidal cells, i.e. hepatocytes. Many activated Kupffer cells were found in the SD. The control groups (Lewis to Lewis and Da to Lewis with immunosuppression - CsA 1mg/kg/BW/day) revealed a normal sinusoidal and hepatocellular ultrastructure with only a few activated Kupffer cells.

Keywords:

Rat liver transplantation, sinusoidal lining cells, electronmicroscopy

Kurztitel:

Weber, T. (1994) TxMed 2:177

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