Transplantationsmedizin

Nr. 2 - 2. Quartal 1994

Glycine Inhibitis Phospholipolysis of Hypoxic Membranes

M. Schilling

Glutathione, like its smallest aminoacid glycine, has been shown to be cyto- and vasculoprotective against hypoxic and ischemic injury in numerous organs and animal models. The exact mechanisms of action are unknown, but have been attributed to a membrane stabilizing effect of glycine. In this study we investigated the effect of glycine on phospholipase induced membrane degradation and fatty acid release from rat hepatocytes and rabbit proximal renal tubules. Glycine 10mM given before and during reperfusion significantly reduced (extracellular and intracellular) PLA2 induced membrane degradation in hepatocytes and renal tubules, as well as arachidonic acid release in renal tubules. Glutamine or alanine given at the same concentration are not protective against PLA2 induced injury. It is concluded that glycine inhibits a hypoxia induced, PLA2 mediated arachidonic acid release. During reperfusion, released arachidonic acid could be metabolized to vasoconstrictive eicosanoids in ischemically damaged kidneys and lead to the observed chances of vasoconstriction and decreased renal blood flow.

Keywords:

Glycine, PLA2, hypoxia, vasoconstriction, gluthatione, renal ischemia

Kurztitel:

Schilling, M. (1994) TxMed 2:140

Dr. M. Schilling
Chirurgische Klinik II
Steinhövelstr. 9
D-89075 Ulm

[Pabst Science Publishers] [Transplantationsmedizin] [Inhalt] [Suchen] [Bestellen]

Pabst Science Publishers, Lengerich, Berlin,
Düsseldorf, Riga, Scottdale AZ (USA), Wien, Zagreb